Utilization Of Alcalase Enzyme In The Production Of Malong Fish Protein Hydrolysate (Congresox Talabon) As An Α-Glucosidase Enzyme Inhibitor
Main Article Content
Abstract
This study aims to determine the best protein hydrolyzate from the enzymatic hydrolysis process of malong fish (Congresox talbon) using different concentrations of the alcalase enzyme. The hydrolysis process was carried out at concentrations of 1.5, 2.0, and 2.5% (w/v protein) at 55oC, pH 7.5, and hydrolysis time of 2 hours. The parameters measured included the degree of hydrolysis, yield, protein analysis using the Lowry method, antioxidants, then protein hydrolyzate with the best concentration was analyzed for total amino acids, and α-glucosidase enzyme inhibitors. The results showed the use of alcalce enzymes with a concentration of 2.5% (w/v); temperature 55oC; pH 7.5; and hydrolysis time of 2 hours is the best treatment which has a yield value of 59.04%, the optimum degree of hydrolysis is 91.58% with an IC50 value of <50 ppm which is 43.95 ppm. The dominant amino acid content is glutamate and leucine, and is able to inhibit the action of the α-glucosidase enzyme at a concentration of 10000 ppm (1.04%).
Downloads
Article Details
Section
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
References
[AOAC] Association of Official Analytical Chemist. 2005. Official methods of analysis of the association of official analytical chemist. Arlington, Virginia USA : AOAC Inc.
Amalia, E. 2007. Pemanfaatan Kerang Hijau (Mytilus Viridis) dalam Pembuatan Hidrolisat Protein MenggunakanEnzim Papain. Sripsi. Program studi teknologi hasil perikanan. Fakultas perikanan dan ilmu kelautan. Institut Pertanian Bogor.
Amiza, M. A., Kong, Y. L., and Faazaz. 2012. Effect of hydrolysis on physicochemical properties of cobia (Rachycentron canadum) frame hydrolysate. Journal International Food Research, 19 (1): 199-206.
Andrade-Cetto, A. Bicerra-Jimenez, J. Cardenaz-Vazquez, R. 2008. Alfa-glucosidase Inhibiting activity of some Mexican Plants used in the treatment of type 2 diabetes. Journal of Ethnopharmacology. 116. 27-32.
Bajorek, S. A., Morello, C. M., 2010. Effects of dietary fiber and low glycemic index diet on glucose control in subjects with type 2 diabetes mellitus. Annals of Pharmacother 44 (11):1786-1792.
Bosenberg, L. H., 2008. The Mechanism of Action of Oral Antidiabetic Drugs: a Review of Recent Literature, The Journal of Endocrinology, Metabolism and Diabetes of South Africa, 13(3): 80-88.
Fatmawati dan Mardiana. 2014. Tepung ikan gabus sebagai sumber protein (Food Supplement).Jurnal Bionature15(1):54-60.
Hasnaliza H. 2010. The effects of enzyme concentration, temperature, and incubation time on nitrogen content and degree of hydrolysis of protein precipitate from cockle (Anadara granosa) meat wash water. International Food Research Journal. 17: 147-152.
Haryati, S dan Agustini, T.W. 2006. The Effect of Different Substitution Meals to Physical and Chemical Quality of Instant Noodles. Journal of Control Development, 11 (1):20-30
He, R., Girgih, A.T., Malomo, S. A, Ju, X. R, Aluko, R.E. 2013. Antioxidant activities of enzymatic rapeseed protein hydrolysates and the membrane ultrafiltration fraction. Journal of Functional Food. 5(1): 219-227.
Jacoeb M, Purwaningsih S, dan Rinto. 2011. Anatomi, komponen bioaktif dan aktivitas antioksidan daun mangrove api-api (Avicenia marina). Jurnal Pengolahan Hasil Perikanan Indonesia. 14(2): 141- 150.
Karnila, R. 2012. Daya Hipoglikemik Hidrolisat, Konsentrat, dan Isolat Protein Teripang Pasir (Holothuria scabra j.) pada Tikus Percobaan. [Disertasi]. Bogor : Sekolah Pascasarjana, Institut Pertanian Bogor.
Kim, E. K. 2013. Marine Protein and Peptides, Biological Activities, Oxford (UK):John Wiley and Sons.
Laksono, U. T., Nurhayati, T., Suptijah, P., Nur’aenah, N., dan Nugroho, T. S. 2019. Karakteristik Ikan Malong (Muraenesox cinerus) sebagai Bahan Baku Pengembangan Produk Diversifikasi. JPHPI, 22(1): 60- 70.
Lakshmanasenthil, S., Vinothkumar, T., Geetharamani, D., Marudhupandi, T., Suja, G., Sindhu, N. S. 2014. Fucoidan—a novel α-amylase inhibitor from Turbinaria ornata with relevance to NIDDM therapy. Biocatalysis and Agricultural Biotechnology 3(3):66–70.
Lee, S. H., Park, M., Heo, S. J., Kang, S. M., Han, J. S., Jeon, Y. J. 2010. Dieckol isolated from Ecklonia cava inhibits α-glucosidase and α-amylase in vitro and alleviates postprandial hyperglycemia in streptozotocininduced diabetic mice. Food and Chemical Toxicology 48(10):2633– 2637.
Lee, S. H., Jeon, Y. J. 2013. Anti-diabetic effects of brown algae derived phlorotannins, marine polyphenols through diverse mechanisms. Fitoterapia 86:129–136.
Li G, Le G, Shi Y, Shrestha S. 2004. Angiotensin i-converting enzyme inhibitory peptides derived from food proteins and their physiological and pharmacological effects. Nutr Res 24:469-486.
Liu Z, Jeppesen PB, Gregersen S, Chen X, Hermansen K. 2008. Dose and glucose dependent effects of amino acids on insulin secretion from isolated mouse islets and clonal INS-1E beta-cells. Rev Diab Studies 5(4): 232-244.
Mayur, B., Sandesh, S., Shruti, S., Sung-Yum, S. 2010. Antioxidant and Alfa-glucosidase inhibitory properties of Carpesium Abrotanoides L. J Med Plants Res 4: 1547-1553. DOI: 10.5897/JMPR10.210.
Nagarani, N., Kumaraguru, A. K. 2013. Evaluation of imflamatory, antidiabetic, cytotoxic activity of Kappahycus alvarezii. International Journal Pharmacy Biology Science 4(1): 921 – 929.
Nurhayati T, Nurjanah, Sanapi CH. 2013. Karakteristik Hidrolisat Protein Ikan Lele Dumbo (Clarias gariepinus). J. Pengolahan Hasil Perikanan Indonesia. Vol. 16, No. 3 Tahun 2013.
Onuh, J. O. 2015. Antyhypertensive and Antioxidant Properties of Chikcen Skin Protein Hydroysates: In vivo, in vitro, and metabolomic studies. Thesis. Faculty Of Grdauate Studies, The University of Manitoba.
Ortiz-Andrade, R. R., Sa´nchez-Salgado, J. C., Navarrete-Va´zquez, G., Webster, S. P., Binnie, M., Garcý´a-Jime´nez, S., Leo´n-Rivera, I., Cigarroa-Va´zquez, P., Villalobos-Molina, R., Estrada-Soto, S. 2008. Antidiabetic and toxicological evaluations of naringenin in normoglycaemic and NIDDM rat models and its implications on extra-pancreatic glucose regulation. Diabetes, Obesity and Metabolism 1(1) :1-8.
Purbasari, D. 2008. Produksi dan Karakterisasi Hidrolisat Protein Dari Kerang Mas Ngur (Atactodea striata).[Skripsi]. Program Studi Teknologi Hasil Perikanan,Fakultas Perikanan dan Ilmu Kelautan, InstitutPertanian Bogor, Bogor.
Roy, M. C., Anguenot, R., Fillion, C., Beaulieu, M., Bérubé, J., Richard, D. 2011. Effect of a commercially-available algal phlorotannins extract on digestive enzymes and carbohydrate absorption in vivo. Food Research International 44 (9):3026–3029.
Salwenee, S., Wan, A. W. M., Mamot, S., maskat, M. Y., dan Ibrahim, S. 2013. Effect of enzyme consentration, temprature, pH, and Time on the degree of hidrolysis of protein extract from fishcera tuna (Euthynnus affinis) by using alcalase. Sains Malaysiana 42(3): 279-287.
Samanarayaka, E. G. P., and Li-Chan, E. C. Y. 2011. Food-derived peptidic antioxidant: a review of their production, assesment and potential applications. Journal of Functional Food. 3:229-254.
Uju, T., Nurhayati, B. Ibrahim, W. Trilaksani & M. Siburian. 2009. Karakterisasi dan recovery protein dari air cucian minced fish dengan membran reverse osmosis. Jurnal Pengolahan Hasil Perikanan Indonesia. 12 (2) :115-127.
Winarno, F. G. 2008. Kimia pangan dan gizi. PT. Gramedia Pustaka Utama. Jakarta. 112 hlm.